The final cut of TMC Podcast #34 is available now with topics timestamped. We covered Tesla's rollercoaster prices, Toyota pushing junk science, Mike's new Model 3, Optimizing track mode for snow driving, FSD V11 apparently coming by the end of this week, and more. You can watch and check out the chat replay on YouTube.
Have you tried to unplug it and replug it? Also, check that it hasn't been removed from audio sources (you can enable/disable audio sources). It's possible the update toggled the USB source off. I'm also on 2022.20.8 without an issue.
You could try low level formatting the USB or scanning it for errors. If it comes back clean, this was just a file system corruption (perhaps done by the car) and you can reuse the drive. If it has persistent errors, then it was just a coincidence that it failed at the same time the software was updated.
Also, keep in mind that USB sticks are not rated to work in the automotive temperature range. They often work fine, until they don't. I strongly recommend using SD Drives (with an adapter) as these are rated for automotive temperatures (very high/low). Cabin temps can easily go beyond 140F in the sun, and the drive internally heats up an additional 10-20F. Here's my list of drives with the temperature ranges listed: Recommended Drives for the Tesla Dashcam – TeslaTap
I've had my 2021 Model Y for a year and I've been using USB Drive to play my favorite playlists from a dozen different USB drive and after the latest update my USB drives are no longer recognized....WHY DID THEY DO THAT???